Protein Method Blocking Buffers
LICORbio Intercept T20 (PBS) Protein-Free Antibody Diluent (500mL)
Intercept T20 (PBS) Protein-Free Antibody Diluent improves the specificity of the primary and secondary antibodies, reducing off-target effects. The diluent contains Intercept (PBS) Protein-Free Blocking Buffer preformulated with Tween 20 for use in a phosphate-buffered saline (PBS) system. This diluent can be used where animal-based products are prohibited. Since there’s no need to mix the diluent yourself, it saves you time and reduces potential variation. Intercept T20 (PBS) Protein-Free Antibody Diluent contains a 0.05% concentration of Tween 20. It contains no sodium azide and is stored at 4 °C.
Quality Biological Inc DPBS with TWEEN 20 without Ca & Mg, 10 Liters
DPBS can be used to provide a buffer system to maintain cell culture media in the physiological pH range of 7.0 to 7.6. ¿ DPBS serves as an irrigating, transporting or diluting fluid for mammalian cells, while maintaining cell tonicity and viability for a limited period of time. ¿ DPBS is also used as a diluent of fresh red blood cells to study the trapping of red blood cell aggregate, to wash the wells of microtiter plates, and to wash cells during preparation and serial transfer. ¿ 1X working solution is recommended ¿ Storage Conditions: 15¿C to 30¿C ¿ Shipping Condition: ambient temperature ¿ Sterile Filtered ¿ Product and/or packaging contains recycled/renewable content ¿ Product and/or packaging is recyclable or reduces waste ¿ Product and/or packaging is safer to human and environmental health ¿ ¿ Max Shelf Life: 36 months
Cell Signaling Technology TrueBlack™ Lipofuscin Autofluorescence Quencher, 1 mL
TrueBlack™ Lipofuscin Autofluorescence Quencher is specifically formulated to reduce lipofuscin autofluorescence in aged human and animal tissue samples. It also minimizes general background fluorescence from sources including collagen, elastin, and red blood cells to enhance image clarity in immunofluorescence protocols. Supplied as a 20X concentrate, this reagent is designed for use in frozen tissue preparations.
- Effectively quenches lipofuscin autofluorescence in tissues such as brain, heart, kidney, and retina
- Reduces general background fluorescence
- Supplied as a 20X concentrate in DMF
- Stable for 12 months when stored at room temperature
- Validated for compatibility with many common counterstains, such as DAPI
LICORbio Intercept (TBS) Protein-Free Blocking Buffer (3x500mL)
Intercept (TBS) Protein-Free Blocking Buffers are formulated to provide highly efficient blocking and low background variability for replicable quantitative Western blots and other immunoassays where animal-based products are prohibited. Intercept (TBS) Protein-Free Blocking Buffer is a ready-to-use blocker formulation in Tris-buffered saline (TBS). It contains no proteins, for assays where animal-based products are prohibited, it can be used with the widest range of targets for Western blot detection, it is ideal for primary antibodies that are optimized for Tris-based buffer systems, it can be used for detection of phosphorylated targets, it has superior performance for alkaline phosphatase detection, and it is an excellent choice for biotin-streptavidin detection. Intercept (TBS) Protein-Free Blocking Buffer is a ready-to-use blocker formulation in TBS and is stored at 4 °C. It contains no sodium azide, making it suitable for both near-infrared or chemiluminescent detection.
Bioworld BBS/Fish Gelatin (10X), 200 mL
BBS/Fish Gelatin 10XBBS Buffered fish gelatin 5% blocks (10X), diluents and coating solutions are supplied as 10X concentrates. Technical Information: pH range: 8.0 (+/- 0.1) formulated in a borate buffered physiologic saline bufferSold as 2 x 100mL or 500mL.
Surmodics Inc Phosphate Buffered Saline/Casein Block & Diluent-5X, 1L
BioFX™ Phosphate Buffered Saline/Casein Block and Diluent-5X Concentrate minimizes non-specific interactions of the secondary reagents with each other and the primary solid phase binding sites. The high blocking efficiency of casein is due to its content of small molecular weight proteins. The solution is ready for dilution to specific application requirements.
Biotium 10X Fish Gelatin Blocking Agent
10X Fish Gelatin Blocking Agent is a solution of cold water fish skin gelatin that can be added to blocking buffers to minimize non-specific antibody binding in immunodetection procedures such as Western Blotting and immunostaining. Unlike BSA or milk, fish gelatin does not contain IgG or serum proteins that could cross-react with mammalian antibodies. Fish gelatin can be used to block non-specific binding sites on positively charged nylon or PVDF membranes or used for blocking and dilution of antibodies during immunostaining procedures.
Santa Cruz Biotechnology UltraCruz Blocking Reagent, 100 mL
Plant protein derived universal antibody diluent and blocking reagent recommended for use in immunohistochemistry (IHC), immunofluorescence (IF), Western blotting (WB), and for other research applications. Animal-free alternative to sera, BSA or non-fat dry milk. Designed to stabilize diluted antibody solutions and minimize background due to non-specific binding in different antibody-based research applications. The UltraCruz Blocking Reagent is especially convenient in multiplex IHC (multiple antigen labeling) applications where antibodies from several different species are used.
Advansta Inc ADVANBLOCK-FLUOR BLOCKING SOL
AdvanBlock-Fluor blocking solution. Optimized block solution for the best fluorescent Western blot signals
Biocare Medical, LLC intelliPATH FLX Peroxidase Blocking Reagent - 20 ml
Peroxidazed Blocking Reagent is a highly stable form of hydrogen peroxide for blocking endogenous peroxidase. It is very effective for blocking non-specific staining in red blood cells. It is non-flammable, safer and less toxic when compared to hydrogen peroxide/methanol formulations.
SCANTIBODIES LABORATORY INC HBR 1, PURIFIED (3KC533) 1.0G
The 3KC533 Heterophilic Blocking Reagent 1, Purified from Scantibodies Laboratory, Inc. contains specific murine immunoglobulins that block the heterophilic interaction by active binding to the heterophilic antibodies, which are capable of cross-linking the capture and the detection antibodies used in the immunoassay, resulting in false positive readings. The attachment of HBR-1 to the heterophilic antibodies blocks this cross-linking, and eliminates the interference caused by the heterophilic antibodies in the humoral fluids. In addition to its active blocking, this product is also characteristic of its passive blockage of the heterophilic interaction as well. The HBR is a liquid reagent with a protein concentration of 20 ± 2 mg/ml. The immunoglobulins are dissolved in a phosphate buffer with a pH of 7.2-7.6. The immunoglobulins in this product are at a purity of greater than or equal to 95%.
SCANTIBODIES LABORATORY INC HETEROPHILIC BLOCKING TUBE (3IX762) 50PK
The 3IX762 HBT (Heterophilic Blocking Tube) from Scantibodies Laboratory, Inc. contains a unique blocking reagent composed of specific binders which inactivate heterophilic antibodies. Once the specific binders have bound to the heterophilic antibodies, the antibodies are no longer able to cause immunoassay interference. The reagent is in the form of a lyophilized pellet at the bottom of the tube and each tube contains enough reagents to inactivate the heterophilic antibodies in 500 μl of sample material. The HBT also allows for the rapid and simple elimination of false positive heterophilic interference in plasma or serum for immunoassays (i.e., FSH, LH, Prolactin, TSH, Ferritin, CEA, AFP, hCG, HBsAg, CK-MB, CA 125, CA 19-9, NSE, etc.).
LICORbio Intercept (TBS) Protein-Free Blocking Buffer (10x500mL)
Intercept (TBS) Protein-Free Blocking Buffers are formulated to provide highly efficient blocking and low background variability for replicable quantitative Western blots and other immunoassays where animal-based products are prohibited. Intercept (TBS) Protein-Free Blocking Buffer is a ready-to-use blocker formulation in Tris-buffered saline (TBS). It contains no proteins, for assays where animal-based products are prohibited, it can be used with the widest range of targets for Western blot detection, it is ideal for primary antibodies that are optimized for Tris-based buffer systems, it can be used for detection of phosphorylated targets, it has superior performance for alkaline phosphatase detection, and it is an excellent choice for biotin-streptavidin detection. Intercept (TBS) Protein-Free Blocking Buffer is a ready-to-use blocker formulation in TBS and is stored at 4 °C. It contains no sodium azide, making it suitable for both near-infrared or chemiluminescent detection.
Teknova 1X PBS with 0.1% Tween 80. 1000mL. Sterile.
Phosphate-buffered saline (PBS) is a salt solution that is commonly used in physiological and biochemical experiments. It can be used to maintain pH neutrality or to buffer against changes in pH in order to provide optimal conditions for cellular growth and function. The buffering range is between pH 6.8 and 7.4.