Protein Method Blocking Buffers
Bioworld BBS/Fish Gelatin (10X), 200 mL
BBS/Fish Gelatin 10XBBS Buffered fish gelatin 5% blocks (10X), diluents and coating solutions are supplied as 10X concentrates. Technical Information: pH range: 8.0 (+/- 0.1) formulated in a borate buffered physiologic saline bufferSold as 2 x 100mL or 500mL.
Boston Bioproducts Inc Non-Fat Powdered Milk (Blotto) - 500g
Non-Fat Powdered Milk (Blotto) - 500g (For Research Use and Further Manufacturing Only)
Alkali Scientific PBS/Tween 20 10X Solution, 500 Milliliters
PBS/Tween 20 10X Solution is a concentrated buffer solution that combines the benefits of phosphate-buffered saline with Tween 20, a non-ionic detergent, to enhance the solubilization of biological molecules. This 500mL solution is commonly used for washing and blocking steps in immunological assays, particularly in enzyme-linked immunosorbent assays (ELISA) and western blotting. The addition of Tween 20 helps to reduce non-specific binding and improve the sensitivity of the assay. The 10X concentration allows for easy dilution to working strength, making this product versatile for various laboratory applications. It is an essential reagent for researchers working with proteins, antibodies, and cell cultures.
Alkali Scientific Dry Powder Milk, 500 G
Dry Powder Milk, 500 G, is a high-quality non-fat milk powder that is commonly used in microbiology and biochemical research. It serves as a nutrient source in the preparation of culture media for growing microorganisms such as bacteria and fungi. The milk powder is particularly important for experiments that involve fermentation, microbial cultivation, and the study of metabolic processes. It is often used in laboratories to prepare nutrient-rich media for routine microbiological testing, ensuring the optimal growth of a wide range of microbial species. The 500g size is ideal for smaller-scale media preparation and can be easily incorporated into custom laboratory formulations.
INNOVEX BIOSCIENCES UNIVERSAL Fc RECEPTORS BLOCKER NON-ANTIBODY BASED 30 ML
Innovex peptide-based Fc Blocker universally blocks all types Fc receptors (I, II, III) of human, mouse & all animal species; Contains NO antibody, Ig or Ig fragments, is used for IHC, IF & flow cytometry.
Quality Biological Inc DPBS with TWEEN 20 without Ca & Mg, 10 Liters
DPBS can be used to provide a buffer system to maintain cell culture media in the physiological pH range of 7.0 to 7.6. ¿ DPBS serves as an irrigating, transporting or diluting fluid for mammalian cells, while maintaining cell tonicity and viability for a limited period of time. ¿ DPBS is also used as a diluent of fresh red blood cells to study the trapping of red blood cell aggregate, to wash the wells of microtiter plates, and to wash cells during preparation and serial transfer. ¿ 1X working solution is recommended ¿ Storage Conditions: 15¿C to 30¿C ¿ Shipping Condition: ambient temperature ¿ Sterile Filtered ¿ Product and/or packaging contains recycled/renewable content ¿ Product and/or packaging is recyclable or reduces waste ¿ Product and/or packaging is safer to human and environmental health ¿ ¿ Max Shelf Life: 36 months
Research Products International Corp TBS with Tween, 20X, 7.4 pH, Sterile Buffered, 1 Liter
TBS with Tween, 20X, 7.4 pH, Sterile Buffered is a commonly used buffer solution in the life science marketplace. TBS stands for Tris-Buffered Saline, and Tween refers to Tween 20, a nonionic detergent.
TBS with Tween is primarily used in various applications such as Western blotting, immunohistochemistry, and antibody-based assays. It serves as a buffer for sample dilution, antibody washing, and blocking steps during these experimental procedures.
The buffer solution is prepared at a 20X concentration, meaning it is twenty times more concentrated than the final desired concentration. This concentrated form allows for efficient storage and transportation.
TBS with Tween, 20X, 7.4 pH, Sterile Buffered is specifically formulated to have a pH of 7.4, which is the physiological pH of many biological systems.
Vector Laboratories Animal-Free Blocker and Diluent (AFB), Ready-to-Use (R.T.U.) 100 ml
R.T.U. Animal-Free Blocker and Diluent is a ready-to-use antibody diluent and blocking reagent that stabilizes diluted antibody solutions and diminishes background due to non-specific binding in immunohistochemical applications. It contains no animal-derived proteins and can be used as an alternative to sera, BSA, casein or non-fat dry milk in situations where these or other traditional blocking agents are ineffective. It is especially convenient for multiplex IHC (multiple antigen labeling) applications where antibodies from several different species are used.
Seracare Life Sciences Inc 10X TBS W/ 0 5% TWEEN 20(TBST)
10X Tris-buffered Saline (TBS) with 0.5% Tween 20 is used as a common Western blot wash solution and general wash buffer in immunoassay applications. Tween 20, a non-ionic detergent, reduces non-specific binding and protein-protein interaction during the wash step in immunoassay procedures.
LAB BIOREAGENTS StrongBlock III | 500mL
Lab Bioreagents Buffers have been developed through the screening of >2000 compounds with the support of machine learning (AI) and optimized through kinetic and dynamic binding tests. StrongBlock (I, II, III) are newly developed blocking buffers that provides exceptional performance and broad compatibility for Western blot, ELISA, IHC and nucleic acid detection assays. The blocking buffer is to block excess binding sites on the micro-plate for reducing non-specific binding signal, by which the assay sensitivity and specificity are improved. As many factors can influence nonspecific binding, there is no single blocking buffer that is ideal for each assay. For example, depending on the recognition of detection probes from different assays, one individual blocking buffer may cross-react with detection probe. StrongBlock (I, II, III) are a variety of buffers designed by different blocker components.
Teknova 1X PBS with 0.1% Tween 80. 1000mL. Sterile.
Phosphate-buffered saline (PBS) is a salt solution that is commonly used in physiological and biochemical experiments. It can be used to maintain pH neutrality or to buffer against changes in pH in order to provide optimal conditions for cellular growth and function. The buffering range is between pH 6.8 and 7.4.
Biotium 10X Fish Gelatin Blocking Agent
10X Fish Gelatin Blocking Agent is a solution of cold water fish skin gelatin that can be added to blocking buffers to minimize non-specific antibody binding in immunodetection procedures such as Western Blotting and immunostaining. Unlike BSA or milk, fish gelatin does not contain IgG or serum proteins that could cross-react with mammalian antibodies. Fish gelatin can be used to block non-specific binding sites on positively charged nylon or PVDF membranes or used for blocking and dilution of antibodies during immunostaining procedures.
Crescent Chemical Co Inc Denhardt's Solution, 50X Concentration, 10mL
Denhardt's Solution, 50X Concentration, 10mL, Molecular Biology Grade, DNase/RNase not detected.Suitable for nucleic acid hybridization. Denhardt's solution is a mixture of blocking agents capable of saturating non-specific binding sites and to be used in membrane-based hybridization protocols. It is recommended for use with nylon membranes. Storage Conditions: -15 °C TO -25 °C